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TCR gamma/delta, PE-Cyanine5, clone: eBioGL3 (GL-3, GL3), eBioscience™

Armenian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 15-5711-81

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15310530

  • £95.25 / 50µg

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Description: The GL3 monoclonal antibody reacts with the gamma delta T-cell Receptor complex (TCR) on all gamma delta TCR bearing T cells, but it does not react with alpha beta TCR. The gamma delta TCR is present on cells in the thymus, epidermis, epithelial lining of the intestine, peritoneal cavity, and lymphoid tissues.Applications Reported: This GL3 (GL-3) antibody has been reported for use in flow cytometric analysis.It is recommended to prestain cells with anti-mouse CD16/32 (cat. 14-0161) to prevent non-specific Fc-mediated binding of the GL3 monoclonal antibody.Applications Tested: This eBioGL3 (GL-3, GL3) antibody has been tested by flow cytometric analysis of mouse lymph node cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.


gamma delta TCR
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Armenian Hamster
Store at 2-8°C. Do not freeze. Light-sensitive material.
Flow Cytometry
GL3), eBioGL3 (GL-3
T-cell receptor gamma, t-cell receptor delta, TCRd, TCRg, TCR delta gamma, TCR-CD3 complex

For Research Use Only.