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G-CSF, eFluor 660, clone: 9B4CSF, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 50-7353-82

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15550037

  • £189.15 / 100µg


Estimated Shipment date
from Supplier 27-06-2018
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Description

Description

Description: This 9B4CSF monoclonal antibody reacts with mouse G-CSF (Granulocyte Colony-Stimulating Factor), a growth factor that stimulates the proliferation, differentiation, and mobilization of hematopoetic stem cells. It is expressed by monocytes, macrophages, and bone marrow stromal cells, and can also be induced in fibroblasts by IL-17A. Unlike GM-CSF and IL-3, which can stimulate cells of multiple lineages, G-CSF activity is limited to neutrophilic granulocytes. G-CSF is essential to the maintenance of neutrophil counts during homeostasis, and low basal levels of the protein are detectable in the serum of healthy individuals. Circulating levels become elevated rapidly upon infection, as G-CSF is also important for the activation and mobilization of mature neutrophils during the innate immune response. G-CSF therapy is commonly used to treat neutropenia following bone marrow transplant or chemotherapy.This 9B4CSF antibody has been demonstrated to cross-react with human G-CSF.Applications Reported: This 9B4CSF antibody has been reported for use in flow cytometric analysis, and intracellular staining followed by flow cytometric analysis.Applications Tested: This 9B2-8°CSF antibody has been tested by intracellular staining followed by flow cytometric analysis of in vitro-cultured and stimulated mouse monocytes/macrophages. This can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

G-CSF
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P09920
C17orf33, CSF3OS, G-CSF, GCSF, MGC45931
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow CytometryþFlow Cytometry
9B4CSF
eFluor 660
Csf3
Conjugated
Csf3
IgG2a, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
12985
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