FR4 Rat anti-Mouse, PE-Cy7, Clone: eBio12A5, eBioscience™

Rat Monoclonal Antibody

Brand: Affymetrix eBioscience

Manufacturer Part Number: 25-5445-82

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.25000kg

Disclaimers: For Research Use Only.

Product Code. 15330970

Quantity Price
1 £ 231.74 / 100µg
EU Stock 1
Add to basket

Description and Specification


Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Monoclonal or Polyclonal Monoclonal
Clone eBio12A5
Primary or Secondary Primary
Concentration 0.2mg/mL
Regulatory Status RUO
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage
Gene Alias Folate receptor 4, FOLR4, FRdelta, FRd
Species Reactivity Mouse
Host Species Rat
Isotype IgG2b
Quantity 100μg
Conjugate PE-Cy7
Format Conjugated
Applications Flow Cytometry
Antigen FR4

The monoclonal antibody eBio12A5 recognizes FR4, also known as Folate receptor 4, FR δ and FBP3 (folate binding protein3). FR4 is a heavily glycosylated 35 kD receptor for folic acid and the physiologic circulating form of the vitamin, N5-methyltetrahydrofolate. Natural T regs have high levels of FR4 and together with CD25 can be used to distinguish natural Tregs, effector T cells, memory-like T cells and naïve T cells. FR4hi CD25hi cells are natural Tregs with high levels of Foxp3. FR4hi CD25lo cells are identified as central memory T cells which upon stimulation can proliferate and produce large amounts of IL-2. In contrast FR4lo CD25hi secrete proinflammatory cytokines such as IFNγ and IL-17 and have been characterized as effector memory T cells.

Based on co-staining studies, the epitopes recognized by eBioTH6 (cat 51-5446) and eBio12A5 are different thereby allowing functional studies with eBioTH6 to be evaluated with eBio12A5.