FR4 Rat anti-Mouse, PE-Cy7, Clone: eBio12A5, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 25-5445-80

25UG ANTI-MOUSE FR4 PE-CY7

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.25000kg



Disclaimers: For Research Use Only.

Product Code. 15320970

Quantity Price
1 £ 132.0 / 25µg
Estimated Shipment date
from Supplier 20-01-2017
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Description and Specification

Specification

Antigen FR4
Applications Flow Cytometry
Clone eBio12A5
Concentration 0.2mg/mL
Conjugate PE-Cy7
Format Conjugated
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Gene Alias Folate receptor 4, FOLR4, FRdelta, FRd
Host Species Rat
Isotype IgG2b
Quantity 25μg
Regulatory Status RUO
Species Reactivity Mouse
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal

The monoclonal antibody eBio12A5 recognizes FR4, also known as Folate receptor 4, FR δ and FBP3 (folate binding protein3). FR4 is a heavily glycosylated 35 kD receptor for folic acid and the physiologic circulating form of the vitamin, N5-methyltetrahydrofolate. Natural T regs have high levels of FR4 and together with CD25 can be used to distinguish natural Tregs, effector T cells, memory-like T cells and naïve T cells. FR4hi CD25hi cells are natural Tregs with high levels of Foxp3. FR4hi CD25lo cells are identified as central memory T cells which upon stimulation can proliferate and produce large amounts of IL-2. In contrast FR4lo CD25hi secrete proinflammatory cytokines such as IFNγ and IL-17 and have been characterized as effector memory T cells.

Based on co-staining studies, the epitopes recognized by eBioTH6 (cat 51-5446) and eBio12A5 are different thereby allowing functional studies with eBioTH6 to be evaluated with eBio12A5.