FR4 Rat anti-Mouse, FITC, Clone: eBio12A5, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 11-5445-80

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.25000kg



Disclaimers: For Research Use Only.

Product Code. 15238239

Quantity Price
1 £ 91.11 / 25µg
Estimated Shipment date
from Supplier 09-05-2017
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Description and Specification

Specification

Species Reactivity Mouse
Quantity 25μg
Monoclonal or Polyclonal Monoclonal
Applications Flow Cytometry
Host Species Rat
Isotype IgG2b
Clone eBio12A5
Antigen FR4
Regulatory Status RUO
Format Conjugated
Conjugate FITC
Gene Alias Folate receptor 4, FOLR4, FRdelta, FRd
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Primary or Secondary Primary
Concentration 0.5mg/mL

The monoclonal antibody eBio12A5 recognizes FR4, also known as Folate receptor 4, FR δ and FBP3 (folate binding protein3). FR4 is a heavily glycosylated 35 kD receptor for folic acid and the physiologic circulating form of the vitamin, N5-methyltetrahydrofolate. Natural T regs have high levels of FR4 and together with CD25 can be used to distinguish natural Tregs, effector T cells, memory-like T cells and naïve T cells. FR4hi CD25hi cells are natural Tregs with high levels of Foxp3. FR4hi CD25lo cells are identified as central memory T cells which upon stimulation can proliferate and produce large amounts of IL-2. In contrast FR4lo CD25hi secrete proinflammatory cytokines such as IFNγ and IL-17 and have been characterized as effector memory T cells.

Based on co-staining studies, the epitopes recognized by eBioTH6 (cat 51-5446) and eBio12A5 are different thereby allowing functional studies with eBioTH6 to be evaluated with eBio12A5.