Foxp3 Rat, eFluor 506, Clone: FJK-16s, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 69-5773-82

100UG Anti-Mouse/Rat Foxp3 eFluor 506

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Disclaimers: For Research Use Only.

Product Code. 15500697

Quantity Price
1 £ 325.2 / 100µg
Estimated Shipment date
from Supplier 20-01-2017
Add to basket


Description and Specification

Specification

Antigen Foxp3
Applications Flow Cytometry (Intracellular Staining)
Clone FJK-16s
Concentration 0.2mg/mL
Conjugate eFluor 506
Format Conjugated
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Gene Alias Forkhead Box P3, Scurfin, JM2, Treg
Host Species Rat
Isotype IgG2a
Quantity 100μg
Regulatory Status RUO
Species Reactivity Bovine, Canine, cat, Mouse, Pig, Rat
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal

The FJK-16s antibody reacts with mouse, rat, dog, porcine, bovine and cat Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in ‘scurfy’ (sf) mice. Constitutive high expression of foxP3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

Immunoblotting with FJK-16s antibody has mapped the epitope to amino acids 75-125 of the mouse Foxp3 protein. In the human, this region has been shown to be alternatively spliced at the mRNA level. Both the alternatively-spliced and non-spliced isoforms are present in the CD4+CD25+ subset of lymphocytes. Preliminary RT-PCR experiments have not revealed this alternatively-spliced isoform in mouse splenocytes, suggesting different gene regulation in the mouse and human.