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Foxp3 Rat anti-Mouse, Rat, PerCP-Cy5.5, Clone: FJK-16s, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 45-5773-80

UNSPSC : 12352200

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15331190

  • £186.31 / 25µg


EU Stock 16
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Disclaimers:

For Research Use Only.


Specifications

Description

The FJK-16s antibody reacts with mouse, rat, dog, porcine, bovine and cat Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in ‘scurfy’ (sf) mice. Constitutive high expression of foxP3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

Immunoblotting with FJK-16s antibody has mapped the epitope to amino acids 75-125 of the mouse Foxp3 protein. In the human, this region has been shown to be alternatively spliced at the mRNA level. Both the alternatively-spliced and non-spliced isoforms are present in the CD4+CD25+ subset of lymphocytes. Preliminary RT-PCR experiments have not revealed this alternatively-spliced isoform in mouse splenocytes, suggesting different gene regulation in the mouse and human.

Intracellular staining of mouse splenocytes with FJK-16s using the PE anti-mouse/rat Foxp3 Staining Set and protocol reveals approximately 2% of total cells in the C57Bl/6 strain and approximately 3-5% in the BALB/c mouse strain. Multicolor flow cytometric analysis demonstrates approximately 90% of the CD4+CD25+ cells and 4% of the CD4+CD25- cells staining with FJK-16s. B220+, CD11b+, CD11c+, and Ly6G/Gr-1+ cells do not show significant co-staining with FJK-16s.

Please see our FAQ regarding the usage of eBioscience Foxp3 reagents.

Please note that FJK-16s has been optimized for use with the Foxp3 Staining Set (cat. 72-5775 or 71-5775 or 77-5775). The use of other fixation and staining buffers is not recommended.
Description & Specifications

Specifications

Foxp3
FJK-16s
PerCP-Cy5.5
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Rat
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry (Intracellular Staining)
0.2mg/mL
Conjugated
Forkhead Box P3, Scurfin, JM2
IgG2a
RUO
Primary
Rat