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FOXP3, eFluor 570, clone: FJK-16s, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 41-5773-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15391070

  • £172.75 / 100µg


EU Stock 1
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Disclaimers:

For Research Use Only.


Description

Description

Description: The FJK-16s antibody reacts with mouse, rat, dog, porcine, bovine and cat Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of foxP3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.Immunoblotting with FJK-16s antibody has mapped the epitope to amino acids 75-125 of the mouse Foxp3 protein. In the human, this region has been shown to be alternatively spliced at the mRNA level. Both the alternatively-spliced and non-spliced isoforms are present in the CD4+CD25+ subset of lymphocytes. Preliminary RT-PCR experiments have not revealed this alternatively-spliced isoform in mouse splenocytes, suggesting different gene regulation in the mouse and human.Applications Reported: This FJK-16s antibody has been reported for use in immunohistochemical staining of frozen tissue sections and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.Applications Tested: This FJK-16s antibody has been tested by immunohistochemistry of frozen mouse spleen at less than or equal to 5 μg/mL. The FJK-16s antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded mouse tissue using high or low pH antigen retrieval and can can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis. Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable.Excitation: 555 nm; Emission: 570 nm
Specifications

Specifications

Foxp3
FJK-16s
eFluor 570
Conjugated
Rat
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Immunohistochemistry (Frozen)
0.2mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Forkhead Box P3, Scurfin, JM2
IgG2a
RUO
Primary
Rat
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