Foxp3 Rat anti-Mouse, PE, Clone: NRRF-30, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 12-4771-80

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.25000kg



Disclaimers: For Research Use Only.

Product Code. 15258779

Quantity Price
1 £ 149.04 / 25µg
Estimated Shipment date
from Supplier 02-05-2017
Add to basket


Description and Specification

Specification

Host Species Rat
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary or Secondary Primary
Quantity 25μg
Gene Alias Forkhead Box P3, Scurfin, JM2, Treg
Isotype IgG2a
Species Reactivity Mouse
Concentration 0.2mg/mL
Regulatory Status RUO
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Monoclonal or Polyclonal Monoclonal
Clone NRRF-30
Conjugate PE
Antigen Foxp3
Format Conjugated
Applications Flow Cytometry (Intracellular Staining)

The NRRF-30 antibody reacts with mouse Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in ‘scurfy’ (sf) mice. Constitutive high expression of foxP3 mRNA has been shown in CD4+/CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+/CD25- cells imparts a Treg phenotype in these cells.

Immunoblotting with NRRF-30 antibody has mapped the epitope to amino acids 1-75 of the mouse Foxp3 protein.

Intracellular staining of mouse splenocytes with fluorochrome-conjugated NRRF-30 using the eBioscience Foxp3 Staining Buffers (cat. 00-5523) and corresponding staining protocol reveals approximately 3% of total cells in the C57Bl/6 strain and approximately 5% in the BALB/c mouse strain. Multicolor flow cytometric analysis demonstrates approximately 90% of the CD4+/CD25+ cells and 4% of the CD4+/CD25- cells staining with NRRF-30. Co-staining with FJK-16s (anti-mouse/rat Foxp3 cat. 71-5775), which has been mapped to amino acids 71-125, and NRRF-30 shows 100% correlation, indicating that the same cells are stained with both anti-mouse Foxp3 antibodies.

Please see our FAQ regarding the usage of eBioscience Foxp3 reagents.