Foxp3 Rat anti-Human, PerCP-Cy5.5, Clone: PCH101, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 45-4776-42

Product Code. 15559036

Disclaimers:

For Research Use Only.

Description

eBioscience offers a panel of monoclonal antibodies to different epitopes of human Foxp3, providing useful tools for investigating the complete expression pattern of Foxp3 at the protein level, and discerning the precise subsets of Foxp3⁺ cells.

The PCH101 antibody reacts with the amino terminus of human foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in ‘scurfy’ (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

Intracellular staining of human peripheral blood mononuclear cells (PBMCs) with PCH101 antibody using the anti-human Foxp3 Staining Set and protocol reveals approximately 0.5-4% of lymphocytes staining, with the majority of staining occurring in the CD25^bright population. This is subject to donor variability.

PCH101 crossreacts with rhesus, chimpanzee and cynomolgus. We recommend the use of CD4 (OKT4, cat. 11-0048, or RPA-T4, cat. 11-0049, depending on the species) and CD25 (BC96, cat. 17-0259).

Specifications

• Antigen: Foxp3
• Clone: PCH101
• Conjugate: PerCP-Cyanine5.5
• Format: Conjugated
• Host Species: Rat
• Quantity: 100 Tests
• Storage Requirements: Store at 2-8°C. Do not freeze. Light-sensitive material.
• Monoclonal or Polyclonal: Monoclonal

• Applications: Flow Cytometry (Intracellular Staining)
• Concentration: 5μL (0.125μg)/test
• Formulation: aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
• Gene Alias: Forkhead Box P3, Scurfin, JM2
• Isotype: IgG2a
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human