Foxp3 Mouse, eFluor 660, Clone: 150D/E4, eBioscience™

Mouse Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 50-4774-41

Code: NEW

Additional Details:
Additional Details: Weight: 0.23750kg



Disclaimers: For Research Use Only.

Product Code. 15599946

Quantity Price
1 £ 74.51 / 25 tests
EU Stock 1
Add to basket


Description and Specification

Specification

Primary or Secondary Primary
Isotype IgG1
Clone 150D/E4
Antigen Foxp3
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Host Species Mouse
Applications Flow Cytometry (Intracellular Staining)
Gene Alias Forkhead Box P3, Scurfin, JM2
Regulatory Status RUO
Species Reactivity Human, Mouse, Rat
Quantity 25 tests
Concentration 5μL (0.06μg)/test
Monoclonal or Polyclonal Monoclonal
Conjugate eFluor 660
Format Conjugated

eBioscience offers a panel of monoclonal antibodies to different epitopes of human/primate and mouse/rat Foxp3 protein, providing useful tools for investigating the complete expression pattern of Foxp3 at the protein level, and discerning the precise subsets of Foxp3+ cells. Other antibodies to Foxp3 available from eBioscience, which have been used significantly in scientific literature, include the anti-human Foxp3 PCH101 (cat. 72-5776) and ebio7979 (cat. 12-7979), and the anti-mouse/rat Foxp3 FJK-16s (cat. 72-5775). The 150D/E4 has been mapped to the splice region in Exon 2 found in human cells. The splicing of this region has not been shown to occur in mouse. Characterization of this antibody to the splice variant has been limited to epitope mapping. It should be noted that other antibodies such as PCH101 and 236A/E7 will recognize both the spliced and full length forms of the Foxp3 protein and stain more intensely than 150D/E4 and FJK-16s in human cells.

The 150D/E4 antibody reacts with mouse/rat/human Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2. Cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in ‘scurfy’ (sf) mice. Constitutive high expression of FoxP3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of Foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.