FOXP3, clone: 150D/E4, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-4774-80
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionConstitutive high expression of FoxP3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of Foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells. Applications Reported: This 150D/E4 antibody has been reported for use in immunoblotting (WB), and immunohistochemical staining. Applications Tested: This 150D/E4 antibody has been tested by immunoblotting of recombit mouse and human Foxp3 protein. The purified format is not recommended for intracellular staining and flow cytometric analysis. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered. FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.
|PBS with 0.09% sodium azide; pH 7.2|
|AIID, DIETER, Forkhead box P3, foxp3, IPEX, JM2, PIDX, SCURFIN, XPID|
|Human, Mouse, Rat|
|Immunohistochemistry, Immunoprecipitation, Western Blot|
|20371, 317382, 50943|
For Research Use Only.