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FOXP3 Monoclonal Antibody (236A/E7), eFluor™ 450, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

£127.00 - £260.00

Specifications

Antigen FOXP3
Clone 236A/E7
Concentration 5 μL/Test
Applications Flow Cytometry
Classification Monoclonal
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Products 2
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Product Code Brand Quantity Price Quantity & Availability  
15589746
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Affymetrix eBioscience
48-4777-41
25 Tests
£127.00
25 tests
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Estimated Shipment: 09-04-2024
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15599746
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Affymetrix eBioscience
48-4777-42
100 Tests
£260.00
100 tests
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Description

Description

Description: The 236A/E7 antibody reacts with human foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells. Intracellular staining and flow cytometric analysis of freshly isolated human peripheral blood mononuclear cells (PBMCs) with the 236A/E7 antibody using the Foxp3 Staining Buffer Set (cat. 00-5523) and protocol reveals staining of the CD4+CD25^bright population. The epitope from 236A/E7 is different from that of PCH101 (cat. 72-5776). Applications Reported: This 236A/E7 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 236A/E7 antibody has been pre-titrated and tested using the Foxp3/Transctiption Factor Staining Buffer Set (cat. 00-5523) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at 5 μL (0.25 μg) per test.

FOXP3 Monoclonal antibody specifically detects FOXP3 in Human, Non-human Primate, Rhesus Monkey samples. It is validated for Flow Cytometry.
Specifications

Specifications

FOXP3
5 μL/Test
Monoclonal
Liquid
RUO
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
AIID; DIETER; forkhead box P3; forkhead box protein P3; Forkhead box protein P3 41 kDa form; Forkhead box protein P3, C-terminally processed; forkhead/winged helix transcription factor 3; Foxp3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; IPEX; JM2; MGC141961; MGC141963; PIDX; regulatory protein Foxp3; RGD1562112; RP23-54C14.1; scurfin; scurfy; sf; XPID
Foxp3
Primary
4° C, store in dark, DO NOT FREEZE!
Foxp3
236A/E7
Flow Cytometry
eFluor 450
Mouse
Human, Non-human Primate, Rhesus Monkey
Q9BZS1
50943, 574303
IgG1 κ
Affinity chromatography
Antibody
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