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FOXP3, Biotin, clone: 236A/E7, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 13-4777-80

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15289809

  • £77.20 / 25µg


Estimated Shipment date
from Supplier 03-07-2018
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Description

Description

Description: The 236A/E7 antibody reacts with human Foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2. Foxp3, a 49-55kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of Foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.Intracellular staining and flow cytometric analysis of freshly isolated human peripheral blood mononuclear cells (PBMCs) with the 236A/E7 antibody using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol reveals staining of the CD4+CD25bright population.The epitope from 236A/E7 is different from that of PCH101 (cat. 72-5776). This antibody has also been shown to recognize rhesus macaque, sooty mangabey and cynomolgus macaque.Applications Reported: This 236A/E7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis and immunohistochemical staining on paraffin and frozen sections.Applications Tested: This 236A/E7 antibody has been tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Buffer Set (cat. 00-5523) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Foxp3
Monoclonal
0.5mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q9BZS1
AIID, DIETER, Forkhead box P3, foxp3, IPEX, JM2, PIDX, SCURFIN, XPID
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
HumanþNon-human PrimateþRhesus
Flow CytometryþImmunohistochemistryþImmunohistochemistry (Frozen)
236A/E7
Biotin
FOXP3
Conjugated
FOXP3
IgG1, kappa
25μg
Store at 2-8°C. Do not freeze.
Primary
50943
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