Foxp3 Mouse anti-Human, APC, Clone: 236A/E7, eBioscience™

Mouse Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 17-4777-42

100TEST Anti-Human Foxp3 APC

Code: NEW

Additional Details:
Additional Details: Weight: 0.23750kg



Disclaimers: For Research Use Only.

Product Code. 15598096

Quantity Price
1 £ 186.31 / 100 tests
EU Stock 12
Add to basket


Description and Specification

Specification

Antigen Foxp3
Gene Alias Forkhead Box P3, Scurfin, JM2
Host Species Mouse
Applications Flow Cytometry (Intracellular Staining)
Isotype IgG1
Quantity 100 tests
Regulatory Status RUO
Species Reactivity Human, Non-human primate, Rhesus Ma
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal or Polyclonal Monoclonal
Format Conjugated
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Primary or Secondary Primary
Clone 236A/E7
Concentration 5μL (0.125μg)/test
Conjugate APC

The 236A/E7 antibody reacts with human Foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2. Foxp3, a 49-55kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in ‘scurfy’ (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of Foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

Intracellular staining and flow cytometric analysis of freshly isolated human peripheral blood mononuclear cells (PBMCs) with the 236A/E7 antibody using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol reveals staining of the CD4+CD25bright population.

The epitope from 236A/E7 is different from that of PCH101 (cat. 72-5776). This antibody has also been shown to recognize rhesus macaque, sooty mangabey and cynomolgus macaque.