Foxp3 Mouse anti-Human, Alexa Fluor 488, Clone: 236A/E7, eBioscience™

Mouse Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 53-4777-41

Code: NEW

Additional Details:
Additional Details: Weight: 0.23750kg



Disclaimers: For Research Use Only.

Product Code. 15580217

Quantity Price
1 £ 92.86 / 25 tests
Estimated Shipment date
from Supplier 04-05-2017
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Description and Specification

Specification

Host Species Mouse
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Species Reactivity Human, Non-human primate, Rhesus Ma
Conjugate Alexa Fluor 488
Concentration 5μL (0.5μg)/test
Primary or Secondary Primary
Isotype IgG1
Regulatory Status RUO
Monoclonal or Polyclonal Monoclonal
Applications Flow Cytometry (Intracellular Staining)
Applications Immunohistochemistry (Formalin/Paraffin)
Applications Immunohistochemistry (Frozen)
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Gene Alias Forkhead Box P3, Scurfin, JM2
Format Conjugated
Clone 236A/E7
Antigen Foxp3
Quantity 25 tests

The 236A/E7 antibody reacts with human Foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2. Foxp3, a 49-55kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in ‘scurfy’ (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of Foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

Intracellular staining and flow cytometric analysis of freshly isolated human peripheral blood mononuclear cells (PBMCs) with the 236A/E7 antibody using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol reveals staining of the CD4+CD25bright population.

The epitope from 236A/E7 is different from that of PCH101 (cat. 72-5776). This antibody has also been shown to recognize rhesus macaque, sooty mangabey and cynomolgus macaque.