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FOXP3 Monoclonal Antibody (236A/E7), Alexa Fluor™ 488, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
£121.00 - £262.00
Specifications
Clone | 236A/E7 |
---|---|
Concentration | 5 μL/Test |
Applications | Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin) |
Classification | Monoclonal |
Conjugate | Alexa Fluor 488 |
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
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Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
15590217
|
Affymetrix eBioscience
53-4777-42 |
100 Tests |
£262.00
100 tests |
In Stock Log in to see stock. |
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15580217
|
Affymetrix eBioscience
53-4777-41 |
25 Tests |
This item is currently unavailable or has been discontinued. View the product page for possible alternatives. |
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Description
Description: The 236A/E7 antibody reacts with human Foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of Foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells. Intracellular staining and flow cytometric analysis of freshly isolated human peripheral blood mononuclear cells (PBMCs) with the 236A/E7 antibody using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol reveals staining of the CD4+CD25bright population. The epitope from 236A/E7 is different from that of PCH101 (cat. 72-5776). This antibody has also been shown to recognize rhesus macaque, sooty mangabey and cynomolgus macaque. Applications Reported: This 236A/E7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis and IHC. Applications Tested: This 236A/E7 antibody has been pre-titrated and tested using the Foxp3/Transctiption Factor Staining Buffer Set (cat. 00-5523) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at 5 μL (0.
FOXP3 Monoclonal antibody specifically detects FOXP3 in Human, Non-human Primate, Rhesus Monkey samples. It is validated for Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin).Specifications
236A/E7 | |
Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin) | |
Alexa Fluor 488 | |
Mouse | |
Human, Non-human Primate, Rhesus Monkey | |
50943, 574303 | |
Primary | |
4° C, store in dark, DO NOT FREEZE! |
5 μL/Test | |
Monoclonal | |
Liquid | |
RUO | |
Q9BZS1 | |
IgG1 κ | |
Affinity chromatography | |
Antibody |
For Research Use Only.