eBioscience™ Fixable Viability Dye eFluor™ 520

Can be used to irreversibly label dead cells prior to cryopreservation, fixation and/or permeabilization procedures. Unlike 7-AAD and PI, cells labeled with Fixable Viability Dyes can be washed, fixed, permabilized and stained for intracellular antigens without loss of staining intensity.

Brand: Affymetrix eBioscience

Manufacturer Part Number: 65-0867-14

Code: NEW

Additional Details:
Additional Details: Weight: 0.23750kg

Disclaimers: For Research Use Only

Product Code. 15580607

Quantity Price
1 £ 48.31 / 100 tests
EU Stock 38
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Description and Specification


Storage Requirements ≤−70°C. Protect from light and moisture.
For Use With (Application) Flow Cytometric Analysis
Quantity 100 tests
Format DMSO, pre-diluted to test size

  • Fixable Viability Dyes may be used to label cells from all species
  • Fixable Viability Dye eFluor™ 520 can be excited by the blue (488nm) laser line and has a peak emission of 522nm that can be detected using a 530/30 band pass filter (equivalent to FITC)
  • For compensation, it is recommended to use a sample of the cells of interest stained with the Fixable Viability Dye
  • Fixable Viability Dye eFluor™ 520 is supplied as a pre-diluted solution prepared in high-quality, anhydrous DMSO. It should be protected from light and moisture
  • Store at −70°C with dessicant
  • It may be freeze-thawed up to 20 times
  • Allow vial to equilibrate to room temperature before opening
  • Staining may be done before or after surface staining
  • Cells may be cryopreserved after staining with no adverse effect on staining intensity of dead cells after thawing