Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 520

Can be used to irreversibly label dead cells prior to cryopreservation, fixation and/or permeabilization procedures. Unlike 7-AAD and PI, cells labeled with Fixable Viability Dyes can be washed, fixed, permabilized and stained for intracellular antigens without loss of staining intensity.

Product Code	Brand	Quantity
15580607	Invitrogen™ 65-0867-14	100 tests
15590607	Invitrogen™ 65-0867-18	500 tests

Description

• Fixable Viability Dyes may be used to label cells from all species
• Fixable Viability Dye eFluor™ 520 can be excited by the blue (488nm) laser line and has a peak emission of 522nm that can be detected using a 530/30 band pass filter (equivalent to FITC)
• For compensation, it is recommended to use a sample of the cells of interest stained with the Fixable Viability Dye
• If the percentage of dead cells is expected to be less than 5%, then it is recommended to take a small aliquot of cells and heat them at 65°C for 1 minute then immediately place on ice for 1 minute. After this treatment, the heat-killed cells can be combined 1:1 with live cells and then stained with the Fixable Viability Dye.
• Fixable Viability Dye eFluor™ 520 is supplied as a pre-diluted solution prepared in high-quality, anhydrous DMSO. It should be protected from light and moisture
• Store at −70°C with dessicant
• It may be freeze-thawed up to 20 times
• Allow vial to equilibrate to room temperature before opening
• Staining may be done before or after surface staining
• Cells may be cryopreserved after staining with no adverse effect on staining intensity of dead cells after thawing

Specifications

• Color: Green
• For Use With (Application): Viability Assay
• Product Type: Dye
• Product Line: eBioscience™

• Detection Method: Fluorescent
• For Use With (Equipment): Flow Cytometer
• Emission: 488⁄522

For Research Use Only. Not for use in diagnostic procedures.