eBioscience Fixable Viability Dye eFluor™ 506

Can be used to irreversibly label dead cells prior to cryopreservation, fixation and/or permeabilization procedures. Unlike 7-AAD and PI, cells labeled with Fixable Viability Dyes can be washed, fixed, permabilized and stained for intracellular antigens without loss of staining intensity.

Overview
Brand: eBioscience

Manufacturer Part Number: 65-0866-18

500TEST Fixable Viability Dye eFluor 506 500 tests

Code: NEW

Additional Details:
Additional Details: Weight: 0.23750kg



Disclaimers: For Research Use Only

Product Code. 15570607

Quantity Price
1 £ 283.2 / 500 tests
Estimated Shipment date
from Supplier 12-12-2016
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Description and Specification

Specification

Quantity 500 tests
Format DMSO, pre-diluted to test size
For Use With (Application) Flow Cytometric Analysis
Storage Requirements ≤−70°C. Protect from light and moisture.

  • Fixable Viability Dyes may be used to label cells from all species
  • Fixable Viability Dye eFluor™ 506 can be excited by the violet (405nm) laser line and has a peak emission of 506nm that can be detected using a 510/50 band pass filter (equivalent to AmCyan)
  • For compensation, it is recommended to use a sample of the cells of interest stained with the Fixable Viability Dye
  • If the percentage of dead cells is expected to be less than 5%, then it is recommended to take a small aliquot of cells and heat them at 65°C for 1 minute then immediately place on ice for 1 minute. After this treatment, the heat-killed cells can be combined 1:1 with live cells and then stained with the Fixable Viability Dye.
  • Fixable Viability Dye eFluor™ 506 is supplied as a pre-diluted solution prepared in high-quality, anhydrous DMSO. It should be protected from light and moisture
  • Store at −70°C with dessicant
  • It may be freeze-thawed up to 20 times
  • Allow vial to equilibrate to room temperature before opening
  • Staining may be done before or after surface staining
  • Cells may be cryopreserved after staining with no adverse effect on staining intensity of dead cells after thawing