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Fisher BioReagents™ Optizyme™ Ribonuclease Inhibitor, Recombinant

Protects RNA from degradation by RNases

Brand:  Fisher BioReagents™ BP3222-1

Code : 32

Additional Details : Weight : 0.00001kg

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Product Code. 10562965

  • £73.65 / 2500 units


Estimated Shipment
14-06-2018
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Description

Description

  • Supplied as a recombinant product from E. coli (originally isolated from rat lung) and provides superior protection of RNA from degradation by RNases
  • Commonly added to all solutions used during the isolation of RNA, and will not interfere with the performance of most enzymes in downstream applications
  • Active over a broad temperature range, and even provides some RNase inhibition at 60°C, which is useful when performing reverse transcription reactions at elevated temperatures to overcome secondary structure in RNA
  • Storage Buffer Components: 20mM HEPES-KOH (pH 7.6 at 4°C), 50mM KCl, 8mM DTT, and 50% glycerol (v/v)

Inactivates RNase A, RNase B, and RNase T2; No activity against SP6, T7, or T3 RNA polymerases, AMV or MMLV reverse transcriptases, and Taq DNA polymerase

  • Displays a broad spectrum of inhibitory activity against RNases, and does not have activity against other polymerases and reverse transcriptases
  • Suitable for use in common molecular biology applications such as isolation and purification of RNA, cDNA synthesis, RT-PCR, in vitro RNA transcription/translation, ribonuclease protection assay, and preparation of RNase-free antibodies

Specifications

Specifications

RNase Inhibitor
20 to 40U/μL
E. coli
Isolation and purification of RNA, cDNA synthesis, RT-PCR, in-vitro RNA transcription/translation, Ribonuclease protection assay and preparation of RNase-free Antibodies
Liquid
Colorless
2,500U
20mM HEPES-KOH (pH 7.6 at 4°C), 50mM KCl, 8mM DTT, 50% Glycerol
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