Fisher BioReagents™ Taq DNA Polymerase

Allows up to 100 cycles of amplification without loss of activity

Overview
Brand: Fisher BioReagents™

Manufacturer Part Number: FB6000-45

X100 UNITS TAQ DNA POLYMERASE, CONCENTRATION 5000units/mL

UNSPSC: 41106314

Code: 32

Additional Details:
Additional Details: CAS Number: 9012-90-2 Weight: 0.00001kg



Product Code. 10449693

Quantity Price
1 £ 49.1 / 100 units
EU Stock 28
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Description and Specification

Specification

For Use With (Application) PCR
Product Name Taq DNA Polymerase
Quantity 100 Units
Format Liquid
Formulation 10X assay buffer B : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 25mM solution in a separate vial
Concentration 5U/μL
Storage Buffer Tris-HCl 20mM, pH 7.5 (at room temperature), KCl 100mM, EDTA 0.1mM, DTT 1mM, Glycerol 50%, Tween™ 20 0.5%, Nonidet™ P40™ 0.5%
Storage Requirements -20°C
Packaging Poly Tube

  • Licensed for use in PCR
  • Uses a thermostable DNA polymerase that has been encoded by a modified form of Thermus aquaticus DNA polymerase gene that has been inserted into an E. coli host
  • Has a 5«→3« exonuclease activity
  • Available with two different reaction buffers
  • Buffer A contains an optimized final concentration of 15mM MgCl2 for most PCR amplifications
  • Buffer B does not contain MgCl2; MgCl2 is provided in a separate vial as a 25mM solution
  • Storage Buffer: 20mM Tris-HCl, pH 7.5 (at room temperature), 100mM KCl, 0.1mM EDTA, 1mM DTT, 50% Glycerol, 0.5% Tween™ 20, 0.5% Nonidet P40™
  • Recommended Assay Buffer: 10X Assay Buffer A (100mM Tris-HCl, pH 9.0 [at 25°C/77°F]; 500mM KCl; 15mM MgCl2) or 10X Assay Buffer B (100mM Tris-HCl, pH 9.0 [at 25°C/77°F]; 500mM KCl) and 25mM MgCl2 solution in a separate vial

Unit Definition: One unit of enzyme is defined as the amount that will incorporate 10nmol of dNTPs into acid-insoluble material per 30 minutes in a 10-minute incubation at 74°C (165°F) under the assay conditions above

Documentation