Fibronectin Monoclonal Antibody (FN-3), Alexa Fluor™ 488, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
15322090	Affymetrix eBioscience 53-9869-80	25 μg
15332090	Affymetrix eBioscience 53-9869-82	100 μg

Description

Description: The FN-3 monoclonal antibody recognizes human fibronectin, a component of the extracellular matrix. This high molecular weight protein (almost 400 kDa) exists as an extracellular and a soluble plasma form. Fibronectin functions in cell adhesion and migration via integrins present on the interacting cell. Fibronectin also associates with collagen, actin and fibrins. In malignancies, fibronectin protein levels can be altered. In lung carninomas, expression is decreased. The FN-3 antibody recognizes a determinant on human cellular but not plasma fibronectin; this recognition is not lost upon trypsin treatment. Furthermore, FN-3 antibody has been shown to crossreact to bovine fibronectin. Applications Tested: This FN-3 antibody has been tested by immunohistochemistry on FFPE human placenta (with IHC Antigen Retrieval Solution - Low pH (cat. 00-4955)) and can be used at less than or equal to 10 μg/mL. Is is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.

Fibronectin Monoclonal antibody specifically detects Fibronectin in Human samples. It is validated for Immunocytochemistry, Immunohistochemistry (Paraffin).

Specifications

• Antigen: Fibronectin
• Concentration: 0.5 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Alias: Anastellin; CIG; Cold insoluble globulin (CIG); Cold-insoluble globulin; cumulus cell-specific fibronectin 1 transcript; DKFZp686F10164; DKFZp686H0342; DKFZp686I1370; DKFZp686O13149; E330027I09; ED-B; embryo-specific fibronectin 1 transcript; ferritin L subunit; ferritin L-chain; FIBNEC; fibronectin; fibronectin 1; fibronectin ED-A- region; fibronectin ED-B+ region; fibronectin variable region; FINC; FN; FN1; fn-1; FNZ; GFND; GFND2; LETS; LOW QUALITY PROTEIN: fibronectin; Migration stimulating factor (MSF); migration-stimulating factor; MSF; Ugl-Y1; Ugl-Y2; Ugl-Y3
• Gene Symbols: FN1
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Gene: FN1

• Clone: FN-3
• Applications: Immunocytochemistry, Immunohistochemistry (Paraffin)
• Conjugate: Alexa Fluor 488
• Host Species: Mouse
• Target Species: Human
• Gene Accession No.: P02751
• Gene ID (Entrez): 2335
• Isotype: IgG1
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.