Thermo Scientific™ XmaJI (AvrII)

Cut at C^CTAGG sites with XmaJI (AvrII) restriction enzyme, which functions best at 37°C in Tango buffer (Isoschizomers: AspA2I, AvrII, BlnI).

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: ER1561

XMAJI (AVRII) 10U/UL 200U

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 1.51950kg



Product Code. 10470090

Quantity Price
1 £ 112.0 / 200 units
EU Stock 26
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Description and Specification

Specification

Product Name XmaJI (AvrII)
Concentration 10U/µL
Units 200U

5'...C▵C T A G G...3'

3'...G G A T C▵C...5'

Conditions for 100% Activity

  • 1X Buffer Tango:33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer

  • XmaJI is supplied in:10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage

  • After 50-fold overdigestion with XmaJI, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects

  • Dam: never overlaps-no effect
  • Dcm: never overlaps-no effect
  • CpG: never overlaps-no effect
  • EcoKI: never overlaps-no effect
  • EcoBI: never overlaps-no effect

Digestion of Agarose-embedded DNA

  • Minimum 10U of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours

Compatible Ends

  • BcuI, NheI, XbaI, Eco130I (C▴CTAGG)