XceI (NspI)

Cut at RCATG^Y sites with XceI (NspI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: BstNSI, NspI).

Overview
Brand: Thermo Scientific

Manufacturer Part Number: ER1472

XCEI (NSPI) 10U/UL 2500U

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.00950kg



Product Code. 10642001

Quantity Price
1 £ 203.0 / 2500 units
EU Stock 2
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Description and Specification

Specification

Product Name XceI (NspI)
Concentration 10U/µL
Units 2,500U

5'...R C A T G▵Y...3'

3'...Y▵G T A C R...5'

Conditions for 100% Activity

  • 1X Buffer Tango: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer

  • XceI is supplied in:10mM Tris-HCl (pH 7.5 at 25°C), 300mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage

  • After 50-fold overdigestion with XceI, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects

  • Dam: never overlaps-no effect
  • Dcm: never overlaps-no effect
  • CpG: may overlap-no effect
  • EcoKI: never overlaps-no effect
  • EcoBI: never overlaps-no effect

Compatible Ends

  • PaeI, Hin1II