5'..C C A NN N N^N T G G...3'
3'..G G T N^N N NN A C C...5'
Conditions for 100% Activity
Ligation and Recleavage
Digestion of Agarose-embedded DNA
Van91I is blocked by overlappingdcm methylation. To avoiddcm methylation, use adam,dcm- strain such as GM2163 (#M0099).