Thermo Scientific™ Van91I (PflMI)

Cut at CCANNNN^NTGG sites with Van91I (PflMI) restriction enzyme, which performs best at 37°C in R buffer (Isoschizomers: AccB7I, BasI, PflMI).

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: ER0711

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 10588930

Quantity Price
1 £ 26.740000000000002 / 400 units
EU Stock 14
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Description and Specification

Specification

Concentration 10U/µL
Units 400U
Product Name Van91I (PflMI)

5'..C C A NN N N^N T G G...3'

3'..G G T N^N N NN A C C...5'

Conditions for 100% Activity

  • 1X Buffer R: 10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl2, 100mM KCl and 0.1mg/ml BSA
  • Incubate at 37°C

Storage Buffer

  • Van91I is supplied in:10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol

Ligation and Recleavage

  • After 50-fold overdigestion with Van91I, more than 90% of DNA fragments can be ligated and recut

Methylation Effects

  • Dam: never overlaps – no effect
  • Dcm: may overlap – blocked
  • CpG: never overlaps – no effect
  • EcoKI: never overlaps – no effect
  • EcoBI: never overlaps – no effect&

Digestion of Agarose-embedded DNA

  • Minimum 5 units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours

Note:

Van91I is blocked by overlappingdcm methylation. To avoiddcm methylation, use adam,dcm- strain such as GM2163 (#M0099).