Thermo Scientific™ Uracil-DNA Glycosylase (UDG, UNG)

Uracil-DNA Glycosylase (UDG, UNG) catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar in DNA and prevents carry-over of DNA in PCR reactions.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: EN0362

MODIFYING ENZYME, URACIL-DNA GLYCOSYLASE (UDG,UNG), 1u/uL,supplied with 2 x 1mL of 10X reaction

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 1.51950kg



Disclaimers: Use of this enzyme in certain applications may be covered by patents and may require a license.

Product Code. 10631041

Quantity Price
1 £ 131.0 / Pack of 5
EU Stock 25
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Description and Specification

Specification

Concentration 1U/┬ÁL
Inhibitors Ugi protein from the Bacillus subtilis phage PBS2, protein p56 from the Bacillus subtilis phage phi29 (7).
Source E. coli K12 cells
Molecular Weight 25.6 kDa monomer
Units 5 x 200U

  • Active in Fermentas buffers for restriction enzymes and thermophilic polymerases
  • Source: E. coli K12 cells
  • Molecular Weight: 25.6 kDa monomer
Quality Control:
  • The absence of endo-, exodeoxyribonucleases, phosphatases and ribonucleases confirmed by appropriate quality tests

Source:

  • E.coli K12 cells

Molecular Weight:

  • 25.6kDa monomer

Definition of Activity Unit:

  • One unit of the enzyme catalyzes the release 1 nanomole of uracil from uracil-containing DNA template in 60 min. at 37°C
Storage Buffer: The enzyme is supplied in:
  • 30mM Tris-HCl (pH 7.5), 150mM NaCl, 1mM EDTA, 1mM DTT, 0.05% (v/v) Tween 20 and 50% (v/v) glycerol

10X Reaction Buffer:

  • 200mM Tris-HCl (pH 8.2 at 25°C), 10mM EDTA, 100mM NaCl
Inhibition and Inactivation:
  • Inhibitors: Ugi protein from the Bacillus subtilis phage PBS2, protein p56 from the Bacillus subtilis phage phi29 (7).
  • Inactivated by heating at 95°C for 10 min. Enzyme activity is partially restored at temperatures lower than 55°C. Therefore put PCR products on ice after PCR and load directly on a gel

Recommended for:

Control of carry-over contamination in PCR (2); Glycosylase mediated single nucleotide polymorphism detection (GMPD) (3); Site-directed mutagenesis (4); As a probe for protein-DNA interaction studies (5); SNP genotyping; Cloning of PCR products (6); Generation of single strand overhangs of PCR products and cDNA

Note:

The abasic sites formed in DNA by Uracil-DNA Glycosylase may be subsequently cleaved by heat, alkali-treatment or endonucleases that cleave specifically at abasic sites.UDG (UNG) is active in the presence or absence of divalent cations.