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Thermo Scientific™ Uracil-DNA Glycosylase (UDG, UNG)

Uracil-DNA Glycosylase (UDG, UNG) catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar in DNA and prevents carry-over of DNA in PCR reactions.

Brand:  Thermo Scientific™ EN0361

Code : 88

Additional Details : Weight : 1.51950kg

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Product Code. 10621041

  • £33.80 / 200 units


EU Stock 63
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Description

Description

  • Active in Fermentas buffers for restriction enzymes and thermophilic polymerases
  • Source: E. coli K12 cells
  • Molecular Weight: 25.6 kDa monomer
Quality Control:
  • The absence of endo-, exodeoxyribonucleases, phosphatases and ribonucleases confirmed by appropriate quality tests

Source:

  • E.coli K12 cells

Molecular Weight:

  • 25.6kDa monomer

Definition of Activity Unit:

  • One unit of the enzyme catalyzes the release 1 nanomole of uracil from uracil-containing DNA template in 60 min. at 37°C
Storage Buffer: The enzyme is supplied in:
  • 30mM Tris-HCl (pH 7.5), 150mM NaCl, 1mM EDTA, 1mM DTT, 0.05% (v/v) Tween 20 and 50% (v/v) glycerol

10X Reaction Buffer:

  • 200mM Tris-HCl (pH 8.2 at 25°C), 10mM EDTA, 100mM NaCl
Inhibition and Inactivation:
  • Inhibitors: Ugi protein from the Bacillus subtilis phage PBS2, protein p56 from the Bacillus subtilis phage phi29 (7).
  • Inactivated by heating at 95°C for 10 min. Enzyme activity is partially restored at temperatures lower than 55°C. Therefore put PCR products on ice after PCR and load directly on a gel

Recommended for:

Control of carry-over contamination in PCR (2); Glycosylase mediated single nucleotide polymorphism detection (GMPD) (3); Site-directed mutagenesis (4); As a probe for protein-DNA interaction studies (5); SNP genotyping; Cloning of PCR products (6); Generation of single strand overhangs of PCR products and cDNA

Note:

The abasic sites formed in DNA by Uracil-DNA Glycosylase may be subsequently cleaved by heat, alkali-treatment or endonucleases that cleave specifically at abasic sites.UDG (UNG) is active in the presence or absence of divalent cations.

Specifications

Specifications

1U/μL
E. coli K12 cells
200U
Ugi protein from the Bacillus subtilis phage PBS2, protein p56 from the Bacillus subtilis phage phi29 (7).
25.6 kDa monomer
Videos

Use of this enzyme in certain applications may be covered by patents and may require a license.