Inhibitors: metal chelators, ammonium, chloride, iodide, phosphate ions
Inactivated by heating at 70°C for 10 min. or by addition of EDTA
Production of synthetic homo- and heteropolymers (1); Homopolymeric tailing of linear duplex DNA with any type of 3'-OH terminus (2, 3); Oligodeoxyribonucleotide and DNA labeling (2, 4-8); 5'-RACE (Rapid Amplification of cDNA Ends) (9); In situ localization of apoptosis (10)
Due to the presence of CoCl2 the TdT Reaction Buffer is incompatible with downstream applications. It is necessary to remove CoCl2 from the reaction mixture by spin column or phenol/chloroform extraction and subsequent ethanol precipitation.