Thermo Scientific™ TaaI (HpyCH4III)

The TaaI (HpyCH4III) restriction enzyme recognizes ACN^GT sites and cuts best at 65°C in Tango buffer (Isoschizomers: Bst4CI, HpyCH4III).

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: ER1361

200 UNIT RESTRICTION ENZYME, CONVENTIONAL, TAAI(HpyCH4III), 10u/ul, cuts ACN¬GT at 65°C (supplied

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.00800kg



Product Code. 10548170

Quantity Price
1 £ 41.56 / 200 units
EU Stock 9
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Description and Specification

Specification

Product Name TaaI (HpyCH4III)
Concentration 10U/µL
Units 200U

5'...A C N▵G T...3'

3'...T G▵N C A...5'

Conditions for 100% Activity

  • 1X Buffer Tango: 33mM Tris-acetate (pH7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 65°C
  • To ensure higher efficiency of digestion, perform the cleavage reaction under paraffin oil

Storage Buffer

  • TaaI is supplied in:10mM Tris-HCl (pH7.5 at 25°C), 100mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage

  • After 50-fold overdigestion with TaaI, approximately 90% of the DNA fragments can be ligated and recut

Methylation Effects

  • Dam: never overlaps — no effect
  • Dcm: never overlaps — no effect
  • CpG: may overlap — cleavage impaired
  • EcoKI: may overlap — effect not determined
  • EcoBI: may overlap — effect not determined

Compatible Ends

  • Hpy188I

Note:

Incubation at 37°C results in 10% activity.