Thermo Scientific™ SspI

The SspI restriction enzyme recognizes AAT^ATT sites and cuts best at 37°C in G buffer.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: ER0771

RESTRICTION ENZYME, CONVENTIONAL, SSPI, 10U/UL,cuts AAT¬ATT at 37°C (suppliedwith 1mL of 10X

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 1.51950kg



Product Code. 10621811

Quantity Price
1 £ 27.22 / 500 units
EU Stock 18
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Description and Specification

Specification

Product Name SspI
Concentration 10U/µL
Units 500U

5'...A A T▵A T T...3'

3'...T T A▵T A A...5'

Conditions for 100% Activity

  • 1X Buffer G:10mM Tris-HCl (pH7.5 at 37°C), 10mM MgCl2, 50mM NaCl and 0.1mg/mL BSA.Incubate at 37°C

Storage Buffer

  • SspI is supplied in:10mM Tris-HCl (pH7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage

  • After 10-fold overdigestion with SspI, more than 90% of the DNA fragments can be ligated and recut

Methylation Effects

  • Dam: never overlaps — no effect
  • Dcm: never overlaps — no effect
  • CpG: never overlaps — no effect
  • EcoKI: never overlaps — no effect
  • EcoBI: may overlap — no effect

Digestion of Agarose-embedded DNA

  • Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours

Note:

Greater than 10-fold overdigestion with SspI may result in star activity.