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Thermo Scientific™ ScaI

The ScaI restriction enzyme recognizes AGT^ACT sites and cuts best at 37°C in its own unique buffer (Isoschizomers: AssI, BmcAI, ZrmI).

Brand:  Thermo Scientific™ ER0431

UNSPSC : 12352204

Code : 88

Additional Details : Weight : 1.51950kg

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Product Code. 10600081

  • £46.95 / 1000 units


EU Stock 19
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Specifications

Description

5'...A G T▵A C T...3'

3'...T C A▵T G A...5'

Conditions for 100% Activity

  • 1X Buffer ScaI:10mM Bis-Tris Propane-HCl (pH6.5 at 37°C), 10mM MgCl2, 100mM KCl and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer

  • ScaI is supplied in:10mM Tris-HCl (pH7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage

  • After 50-fold overdigestion with ScaI, more than 80% of the DNA fragments can be ligated and more than 90% of these can be recut

Methylation Effects

  • Dam: never overlaps — no effect
  • Dcm: never overlaps — no effect
  • CpG: never overlaps — no effect
  • EcoKI: never overlaps — no effect
  • EcoBI: may overlap — blocked

Digestion of Agarose-embedded DNA

  • Minimum 20units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours

Note:

Conditions of low salt, high enzyme concentration, glycerol concentration > 5% or pH> 8.0 may result in star activity. Supercoiled plasmids may require up to 20-fold more ScaI for complete digestion than linear DNA.

Description & Specifications

Specifications

10U/┬ÁL
1,000U
ScaI