Thermo Scientific™ SatI (Fnu4HI)

The SatI (Fnu4HI) restriction enzyme recognizes GC^NGC sites and cuts best at 37°C in G buffer (Isoschizomers: Fnu4HI, Fsp4HI, ItaI).

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: ER1641

SATI (FNU4HI) 10U/UL 200U

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 10558170

Quantity Price
1 £ 51.75 / 200 units
EU Stock 17
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Description and Specification

Specification

Product Name SatI (Fnu4HI)
Concentration 10U/┬ÁL
Units 200U

5'...G C▵N G C...3'

3'...C G N▵C G...5'

Conditions for 100% Activity

  • 1X Buffer G:10mM Tris-HCl (pH7.5 at 37°C), 10mM MgCl2, 50mM NaCl and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer

  • SatI is supplied in:10mM Tris-HCl (pH7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage

  • After 50-fold overdigestion with SatI, more than 60% of the DNA fragments can be ligated in a reaction mixture containing 10-30U of T4 DNA Ligase/1μg of fragments and 10% PEG
  • More than 90% of these can be recut

Methylation Effects

  • Dam: never overlaps — no effect
  • Dcm: never overlaps — no effect
  • CpG: may overlap — blocked
  • EcoKI: never overlaps — no effect
  • EcoBI: never overlaps — no effect

Compatible Ends

  • GC^SGC — BcnI, Bme1390I
  • GC^WGC — MvaI, Bme1390I

Note:

At least two copies of SatI (Fnu4HI) recognition site are required for efficient cleavage.