Thermo Scientific™ RseI (MslI)

The RseI (MslI) restriction enzyme recognizes CAYNN^NNRTG sites and cuts best at 37°C in R buffer (Isoschizomers: MslI, SmiMI).

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: ER2002

RSEI (MSLI) 10U/UL 1000U

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 10850241

Quantity Price
1 £ 105.0 / 1000 units
Estimated Shipment date
from Supplier 07-12-2016
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Description and Specification

Specification

Product Name RseI (Msll)
Concentration 10U/µL
Units 1,000U

5'...C A Y N N▵N N R T G...3'

3'...G T R N N▵N N Y A C...5'

Conditions for 100% Activity

  • 1X Buffer R:10mM Tris-HCl (pH8.5 at 37°C), 10mM MgCl2, 100mM KCl and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer

  • RseI is supplied in:10mM Tris-HCl (pH7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage

  • After 50-fold overdigestion with RseI, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: may overlap —no effect
  • EcoKI: may overlap —blocked
  • EcoBI: may overlap —effect not determined

Digestion of Agarose-embedded DNA

  • Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours