Thermo Scientific™ RNase H

Ribonuclease H (RNase H) is an endoribonuclease that specifically degrades the RNA strand in RNA-DNA hybrids.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: EN0202

MODIFYING ENZYME, RNASE H, 5U/UL, SUPPLIED WITH1mL of 10X reaction buffer 500 units Store at

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 1.51950kg



Product Code. 10519310

Quantity Price
1 £ 477.0 / 500 units
EU Stock 10
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Description and Specification

Specification

Concentration 5U/┬ÁL
Inhibitors metal chelators, SH-blocking reagents
Source E.coli MRE-600 cells
Molecular Weight 18.4kDa monomer
Units 500U

  • Does not hydrolyze the phosphodiester bonds within single-stranded and double-stranded DNA and RNA
Quality Control:
  • The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests

Source:

  • E.coli MRE-600 cells

Molecular Weight:

  • 18.4kDa monomer

Definition of Activity Unit:

  • One unit of the enzyme catalyzes the formation of 1nmol of acid soluble products in 20 min. at 37°C
  • Enzyme activity is assayed in the following mixture: 20mM Tris-HCl (pH 7.8), 40mM KCl, 8mM MgCl2, 1mM DTT, 24μM [3H]-poly(A)·poly(dT), 0.03mg/mL BSA, 4% (v/v) glycerol
Storage Buffer:
  • The enzyme is supplied in: 25mM HEPES-KOH (pH 8.0), 50mM KCl, 0.1mM EDTA, 1mM DTT, 0.1mg/mL BSA and 50% (v/v) glycerol

10X Reaction Buffer:

  • 200mM Tris-HCl (pH 7.8), 400mM KCl, 80mM MgCl2, 10mM DTT

Inhibition and Inactivation:

  • Inhibitors: metal chelators, SH-blocking reagents
  • Inactivated by heating at 65°C for 10 min.

Recommended for:

  • Removal of mRNA prior to synthesis of second strand cDNA (1)
  • RT-PCR and qRT-PCR: removal of RNA after first strand cDNA synthesis
  • Removal of the poly(A) sequences of mRNA after hybridization with oligo(dT) (2)
  • Site-specific cleavage of RNA (3)
  • Studies of in vitro polyadenylation reaction products