Thermo Scientific™ RevertAid™ Reverse Transcriptase

Perform efficient synthesis of full-length first strand cDNA up to 13 kb with this genetically modified M-MuLV RT for routine reverse transcription.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: EP0441

REVERTAID RT 200U/UL 10000U

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 1.51950kg



Product Code. 10612191

Quantity Price
1 £ 46.92 / 10000 units
EU Stock 90
Add to basket


Description and Specification

Specification

For Use With (Application) First strand cDNA synthesis for RT-PCR and real-time RT-PCR; Synthesis of cDNA for cloning and expression; Generation of labeled cDNA probes for microarrays; DNA labeling (3)
Includes 5 x 1mL of 5X Reaction Buffer
Product Name RevertAid Reverse Transcriptase
Quantity 100U

The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids which is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase (1, 2).
  • Efficient synthesis of full-length first strand cDNA up to 13kb
  • Optimum activity at 42°C
  • Active up to 50°C
  • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, fluorescein-labeled nucleotides)

Recommended for:

First strand cDNA synthesis for RT-PCR and real-time RT-PCR; Synthesis of cDNA for cloning and expression; Generation of labeled cDNA probes for microarrays; DNA labeling (3)