Thermo Scientific™ RevertAid™ Premium First Strand cDNA Synthesis Kit

Perform efficient synthesis of first strand cDNA from RNA templates with this complete synthesis system.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: K1621

20 RXN REVERTAID(TM) 1ST STRAND CDNA SYNTHESISkit, includes RevertAid RT, RiboLock, buffer,

UNSPSC: 41106615

Code: 88

Additional Details:
Additional Details: Weight: 2.17720kg



Product Code. 10680471

Quantity Price
1 £ 63.0 / 20 reactions
EU Stock 71
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Description and Specification

Specification

For Use With (Application) First strand cDNA synthesis for RT-PCR and real-time RT-PCR (1, 2); Construction of full length cDNA libraries; Generation of probes for hybridization; aRNA synthesis.
Product Type RevertAid™
Sufficient For 20 reactions

The kit uses RevertAid Reverse Transcriptase which has lower RNase H activity, compared to AMV reverse transcriptase. The recombinant RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA template from degradation. It is fully compatible with reverse transcription reaction, as it maintains activity at temperatures up to 55°C. The oligo(dT)18 anneals selectively on the poly(A) tail of mRNA. Random hexamer primers do not require the presence of poly(A). Therefore, they can be used for transcription of the 5'-end regions of mRNA or cDNA synthesis using RNA without poly(A) tail, e.g., micro RNAs. Gene-specific primers may also be used with the kits. The first strand of cDNA can be directly used as a template in PCR (see RT-PCR protocol), real-time PCR or in second strand cDNA synthesis (see protocol).
  • Full-length first strand cDNA up to 13kb
  • Increased reaction temperatures in the range of 42° to 50°C
  • Supplied with the recombinant RiboLock RNase Inhibitor
  • Complete—oligo(dT)18 and random hexamer primers included with the kit

Recommended for:

First strand cDNA synthesis for RT-PCR and real-time RT-PCR (1, 2); Construction of full length cDNA libraries; Generation of probes for hybridization; aRNA synthesis.