Thermo Scientific™ Rapid DNA Ligation Kit

Enable fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: K1422

RAPID DNA LIGATION KIT 50RXN

UNSPSC: 41106617

Code: NEW

Additional Details:
Additional Details: Weight: 1.58760kg



Product Code. 10775841

Quantity Price
1 £ 117.0 / 50 reactions
EU Stock 56
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Description and Specification

Specification

For Use With (Application) Routine cloning experiments; Blunt-end cloning; Library construction; TA cloning.
Product Type Rapid DNA Ligation Kit
Quantity 50 reactions

The kit contains T4 DNA ligase and a specially formulated 5X rapid ligation buffer optimized for fast and efficient DNA ligation. Fast ligation efficiency is equal to that obtained with T4 DNA ligase in a standard 1 hour ligation. The ligation reaction mixture can be used directly for bacterial transformation with the TransformAid Bacterial Transformation Kit or with other conventional transformation procedures.
  • Fast—ligation of either sticky- or blunt-end DNA in only 5 minutes
  • Convenient—reaction mixture can be used directly for bacterial transformation

Recommended for:

Routine cloning experiments; Blunt-end cloning; Library construction; TA cloning.

Note:

Lyophilized bacterial strains GM2163 (†M0099) and JM107 (†M0109) can be provided free of charge upon request, with any purchase. Prior to electroporation it is necessary to inactivate T4 DNA ligase (1) by chloroform extraction.