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Thermo Scientific™ Psp1406I (AclI)

The Psp1406I (AclI) restriction enzyme recognizes AA^CGTT sites and cuts best at 37°C in Tango buffer (Isoschizomers: AclI).

Brand:  Thermo Scientific™ ER0942

Code : 88

Additional Details : Weight : 0.00900kg

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Product Code. 10734681

  • £212.89 / 1500 units

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5'..A A▵C G T T...3'

3'..T T G C▵A A...5'

Conditions for 100% Activity

  • 1X Buffer Tango:33mM Tris-acetate (pH7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer

  • Psp1406I is supplied in:10mM Tris-HCl (pH7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage

  • After 50-fold overdigestion with Psp1406I, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: completely overlaps —blocked
  • EcoKI: may overlap —effect not determined
  • EcoBI: may overlap —effect not determined

Digestion of Agarose-embedded DNA

  • Minimum 10units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours

Compatible Ends

  • Bsp119I, Bsu15I, Hin1I, Hin6I, HpaII, MspI, NarI, SsiI, TaqI, XmiI


Psp1406I (AclI)