Thermo Scientific™ Psp1406I (AclI)

The Psp1406I (AclI) restriction enzyme recognizes AA^CGTT sites and cuts best at 37°C in Tango buffer (Isoschizomers: AclI).

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: ER0942

PSP1406I (ACLI) 10U/UL 1500U

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.00900kg



Product Code. 10734681

Quantity Price
1 £ 198.0 / 1500 units
Estimated Shipment date
from Supplier 07-12-2016
Add to basket


Description and Specification

Specification

Product Name Psp1406I (AclI)
Concentration 10U/µL
Units 1,500U

5'..A A▵C G T T...3'

3'..T T G C▵A A...5'

Conditions for 100% Activity

  • 1X Buffer Tango:33mM Tris-acetate (pH7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer

  • Psp1406I is supplied in:10mM Tris-HCl (pH7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage

  • After 50-fold overdigestion with Psp1406I, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: completely overlaps —blocked
  • EcoKI: may overlap —effect not determined
  • EcoBI: may overlap —effect not determined

Digestion of Agarose-embedded DNA

  • Minimum 10units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours

Compatible Ends

  • Bsp119I, Bsu15I, Hin1I, Hin6I, HpaII, MspI, NarI, SsiI, TaqI, XmiI