Thermo Scientific™ PscI (PciI)

The PscI (PciI) restriction enzyme recognizes A^CATGT sites and cuts best at 37°C in Tango buffer (Isoschizomers: PciI).

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: ER1872

RESTRICTION ENZYME, CONVENTIONAL, PSCI (PCII),10u/ul, cuts A¬CATGT at 37°C (supplied with 1mL

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.00840kg



Product Code. 10860041

Quantity Price
1 £ 250.0 / 1000 units
EU Stock 6
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Description and Specification

Specification

Product Name PscI (PciI)
Concentration 10U/µL
Units 1,000U

5'...A▵C A T G T...3'

3'...T G T A C▵A...5'

Conditions for 100% Activity

  • 1X Buffer Tango:33mM Tris-acetate (pH7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer

  • PscI is supplied in:10mM Tris-HCl (pH7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage

  • After 50-fold overdigestion with PscI, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: never overlaps —no effect
  • EcoKI: may overlap —effect not determined
  • EcoBI: may overlap —effect not determined

Digestion of Agarose-embedded DNA

  • Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours

Compatible Ends

  • AflIII, BtgI, Eco130I, FatI, NcoI, PagI

Note:

PscI, like its isoschizomers BspLU11I and PciI, is inhibited by nonionic detergents Triton X-100 (>0.002%) and Nonidet P40 (>0.001%).