5'...G C C▵G G C...3'
3'...C G G▵C C G...5'
Conditions for 100% Activity:
Ligation and Recleavage:
Digestion of Agarose-embedded DNA:
Certain sites in pBR322 are difficult to cleave with PdiI, the same as with its prototype NaeI
Assayed using pBR322 DNA (#SD0041)