Thermo Scientific™ PdiI (NaeI)

The PdiI (NaeI) restriction enzyme recognizes GCC^GGC sites and cuts best at 37°C in Tango buffer (Isoschizomers: MroNI, NaeI, NgoMIV).

Brand: Thermo Scientific™

Manufacturer Part Number: ER1521

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 1.51950kg

Product Code. 10784681

Quantity Price
1 £ 21.41 / 200 units
EU Stock 8
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Description and Specification


Concentration 10U/µL
Units 200U
Product Name PdiI (NaeI)

5'...G C C▵G G C...3'

3'...C G G▵C C G...5'

Conditions for 100% Activity:

  • 1X Buffer Tango: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • PdiI is supplied in: 10mM Tris-HCl (pH 7.5 at 25°C), 500mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA, 0.15% Triton X-100 and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with PdiI, more than 90% of the DNA fragments can be ligated and recut

Methylation Effects:

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: completely overlaps —blocked
  • EcoKI: never overlaps —no effect
  • EcoBI: never overlaps —no effect

Digestion of Agarose-embedded DNA:

  • Minimum 20units of the enzyme are required for complete digestion of 1μg of agarose-embedded pBR322 DNA in 16hours


Certain sites in pBR322 are difficult to cleave with PdiI, the same as with its prototype NaeI

Assayed using pBR322 DNA (#SD0041)