Thermo Scientific™ PagI (BspHI)

The PagI (BspHI) restriction enzyme recognizes T^CATGA sites and cuts best at 37°C in O buffer (Isoschizomers: BspHI, CciI, RcaI).

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: ER1282

RESTRICTION ENZYME, CONVENTIONAL, PAGI (BSPHI),10u/ul, cuts T¬CATGA at 37°C (supplied with 1mL

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.00900kg



Product Code. 10679510

Quantity Price
1 £ 193.0 / 2000 units
EU Stock 6
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Description and Specification

Specification

Product Name PagI (BspHI)
Concentration 10U/µL
Units 2,000U

5'...T▵C A T G A...3'

3'...A G T A C▵T...5'

Conditions for 100% Activity:

  • 1X Buffer O: 50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 100mM NaCl and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • PagI is supplied in: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with PagI, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects:

  • Dam: may overlap —cleavage impaired
  • Dcm: never overlaps —no effect
  • CpG: never overlaps —no effect
  • EcoKI: never overlaps —no effect
  • EcoBI: may overlap —cleavage impaired

Digestion of Agarose-embedded DNA:

  • Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours

Compatible Ends:

  • AflIII, PscI, DsaI, Eco130I, NcoI

Note:

Low salt, high glycerol (>5%) concentrations, pH >8.0 or a large excess of enzyme may result in star activity. PagI cleavage is impaired by overlapping dam methylation. To avoid dam methylation, use a dam-, dcm strain such as GM2163 (#M0099).