Thermo Scientific™ PaeI (SphI)

The PaeI (SphI) restriction enzyme recognizes GCATG^C sites and cuts best at 37°C in B buffer (Isoschizomers: BbuI, SphI).

Brand: Thermo Scientific™

Manufacturer Part Number: ER0601


UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.01000kg

Product Code. 10691041

Quantity Price
1 £58.25 / 500 units
EU Stock 28
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Description and Specification


Product Name PaeI (SphI)
Concentration 10U/µL
Units 500U

5'...G C A T G▵C...3'

3'...C▵G T A C G...5'

Conditions for 100% Activity:

  • 1X Buffer B: 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2 and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • PaeI is supplied in: 10mM potassium-phosphate (pH 7.0 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton X-100, 0.5mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with PaeI, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects:

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: may overlap —no effect
  • EcoKI: never overlaps —no effect
  • EcoBI: may overlap —blocked

Digestion of Agarose-embedded DNA:

  • Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours

Compatible Ends:

  • Hin1II, XceI