Thermo Scientific™ PacI

The PacI restriction enzyme recognizes TTAAT^TAA sites and cuts best at 37°C in its own unique buffer.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: ER2201

250 UNIT RESTRICTION ENZYMES, ,conventional, PacI, 10u/uL, cuts TTAAT¬TAA at

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 1.51950kg



Disclaimers: This product or portions thereof is manufactured and sold under license under US. Patent Nos. 5,874,259 and 6,472,177 and other foreign patents.

Product Code. 10809000

Quantity Price
1 £ 59.25 / 250 units
EU Stock 16
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Description and Specification

Specification

Product Name PacI
Concentration 10U/µL
Units 250U

5'...T A A T▵T A A...3'

3'...A T▵T A A T T...5'

Conditions for 100% Activity:

  • 1X Buffer PacI: 10mM Bis-Tris Propane-HCl (pH 6.5 at 37°C), 10mM MgCl2 and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • PacI is supplied in: 10mM potassium phosphate (pH 7.5 at 25°C), 200mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with PacI, more than 95% of the DNA fragments can be ligated and more than 95% of these can be recut

Methylation Effects:

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: never overlaps —no effect
  • EcoKI: may overlap —effect not determined
  • EcoBI: never overlaps —no effect

Digestion of Agarose-embedded DNA:

  • Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded pJET1 DNA with inserted PacI recognition sequence in 16 hours

Compatible Ends:

  • BstKTI, SfaNI (AsiSI), PvuI