5'..G C▵G G C C G C...3'
3'..C G C C G G▵C G...5'
Conditions for 100% Activity:
Ligation and Recleavage:
Digestion of Agarose-embedded DNA:
Assayed using pTZ19RJL2 plasmid DNA digested with BseLI. Supercoiled plasmids may require up to 5-fold more NotI for complete digestion than linear DNAs.