Thermo Scientific™ MunI (MfeI)

The MunI (MfeI) restriction enzyme recognizes C^AATTG sites and cuts best at 37°C in G buffer (Isoschizomers: MfeI).

Brand: Thermo Scientific™

Manufacturer Part Number: ER0751


UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.01000kg

Product Code. 10538160

Quantity Price
1 £29.13 / 300 units
EU Stock 12
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Description and Specification


Product Name MunI (Mfel)
Concentration 10U/µL
Units 300 U

5'...C▵A A T T G...3'

3'...G T T A A▵C...5'

Conditions for 100% Activity:

  • 1X Buffer G: 10mM Tris-HCl (pH7.5 at 37°C), 10mM MgCl2, 50mM NaCl and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • MunI is supplied in: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: never overlaps —no effect
  • EcoKI: never overlaps —no effect
  • EcoBI: may overlap —effect not determined

Digestion of Agarose-embedded DNA:

  • Minimum 5U of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours

Compatible Ends:

  • EcoRI, TasI, XapI