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Thermo Scientific™ MunI (MfeI)

The MunI (MfeI) restriction enzyme recognizes C^AATTG sites and cuts best at 37°C in G buffer (Isoschizomers: MfeI).

Brand:  Thermo Scientific™ ER0752

UNSPSC : 12352204

Code : 88

Additional Details : Weight : 0.01000kg

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Product Code. 10470050

  • £128.00 / 1500 units


Estimated Shipment
07-09-2017
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Description

Description

5'...C▵A A T T G...3'

3'...G T T A A▵C...5'

Conditions for 100% Activity:

  • 1X Buffer G: 10mM Tris-HCl (pH7.5 at 37°C), 10mM MgCl2, 50mM NaCl and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • MunI is supplied in: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: never overlaps —no effect
  • EcoKI: never overlaps —no effect
  • EcoBI: may overlap —effect not determined

Digestion of Agarose-embedded DNA:

  • Minimum 5U of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours

Compatible Ends:

  • EcoRI, TasI, XapI
Specifications

Specifications

MunI (Mfel)
10U/µL
1,500 U