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Thermo Scientific™ MnlI

The MnlI restriction enzyme recognizes CCTC(7/6)^ sites and cuts best at 37°C in G buffer.

Brand:  Thermo Scientific™ ER1071

Code : 88

Additional Details : Weight : 0.01000kg

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Product Code. 10705461

  • £36.62 / 300 units

EU Stock 16
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5'...C C T C (N)7▵...3'

3'...G G A G (N)6▵...5'

Conditions for 100% Activity:

  • 1X Buffer G: 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 50mM NaCl and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • MnlI is supplied in: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: may overlap —no effect
  • EcoKI: never overlaps —no effect
  • EcoBI: may overlap —blocked


MnlI produces DNA fragments that have a single-base 3 extension which are more difficult to ligate than blunt-ended fragments. MnlI may remain associated with the cleaved DNA. This may cause DNA band shifting during electrophoresis. To avoid atypical DNA band patterns, use the 6X DNA Loading Dye & SDS Solution for sample preparation or heat the digested DNA in the presence of SDS prior to electrophoresis.