Thermo Scientific™ MluI

The MluI restriction enzyme recognizes A^CGCGT sites and cuts best at 37°C in R buffer.

Brand:  Thermo Scientific™ ER0561

Product Code. 10540651

Description

5'...A▵C G C G T...3'

3'...T G C G C▵A...5'

Conditions for 100% Activity:

• 1X Buffer R: 10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl₂, 100mM KCl and 0.1mg/mL BSA
• Incubate at 37°C

Storage Buffer:

• MluI is supplied in: 10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

• Dam: never overlaps —no effect
• Dcm: never overlaps —no effect
• CpG: completely overlaps —blocked
• EcoKI: may overlap —no effect
• EcoBI: may overlap —no effect

Digestion of Agarose-embedded DNA:

• Minimum 5U of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours

Compatible Ends:

• AflIII, BtgI, PauI, SgsI

Specifications

• Product Name: MluI
• Concentration: 10U/µL
• Units: 1,000U